Calcium techniques : a laboratory manual /
by Parys, Jan B; Bootman, Martin D; Yule, David I; Bultynck, Geert.
Material type:
BookDescription: xvi, 592 pages : illustrations (some color) ; 28 cm.ISBN: 9781621820789 (hbk.); 1621820785 (hbk.); 9781936113583 (pbk.); 1936113589 (pbk.).Subject(s): Calcium | Calcium Channels | Calcium Signaling | FluorometryDDC classification: 572 INS 1990 C161 Or. Summary: "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
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សៀវភៅភាសាអង់គ្លេស
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Library Block C C161 | 572 INS 1990 C161 Or. (Browse shelf) | Available |
"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
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